The framework consists of about twenty different command-line applications for simulation, exploration, parameter fitting, statistical analysis and other purposes. Therefore, computer simulation is necessary.ฤก3CFLUX2 is a simulator framework for the quantification of metabolic fluxes from experimental measurement data with high-performance computational methods. By labeling the input substrates with isotopes and by measuring the labeling enrichment of the metabolites all over the network with highly sensitive mass spectrometry devices the intracellular flux distribution can be estimated. A widely established tool to quantify fluxes is the isotope labeling experiment. The flux rates in a cell cannot directly be measured. Metabolic flux analysis aims at identification and quantification of intracellular fluxes (i.e. Sampling and Stoichiometry Initialization
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